Spectral Properties of Liquid Samples of Rhodamine (3GO) Dye Disolved in Chloroform

Abstract

This research is focused on the spectral properties (absorption and fluorescence) for Rhodamine 3GO soluble it in Chloroform, at different concentrations (1×10-5 , 2×10-5, 5×10-5, 7×10-5  and 1×10-4 mol/l) at room temperature. The intensity of absorption increased and fluorescence decreased while concentration increased which in agreement with Beer – Lambert Law. The absorption spectrum has been observed which take a wide spectral range, so when the concentration increased each peaks shifted to a long wavelength for each solution. The fluorescence spectrum followed and entirely the same as absorption spectrum, so increasing the concentration shifted peaks to a long wavelength too.  The quantum efficiency of the dissolved Rhodamine 3GO in chloroform was calculated for the same above concentration were (96%, 89%, 87%, 73%, 67%) respectively. In addition the radiative life time was (0.218, 0.496, 1.204, 1.513, 1.536 ns) and the fluorescence life time was (0.207, 0.481, 1.144, 1.225, 1.044 ns) respectively
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