Study of Morphology, Estability and Structure of Burkholderia cepacia Lipase with Alginate Gels

Giovana S. Padilha(1*), Ranulfo M. Alegre(2), Elias B. Tambourgi(3)

(1) State University of Campinas, School of Food Engineering. Monteiro Lobato Street, 80 – CEP: 13083-862 - Campinas/SP - Brazil, Brazil
(2) State University of Campinas, School of Chemical Engineering. Av. Albert Einstein, 500 - CEP 13083-852 – Campinas/SP - Brazil., Brazil
(3) State University of Campinas, School of Food Engineering. Monteiro Lobato Street, 80 – CEP: 13083-862 - Campinas/SP - Brazil, Brazil
(*) Corresponding author


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Abstract


In this work, for enzyme production, the Burkholderia cepacia was grown in medium containing salts, yeast extract, bacteriological peptone and 6% soybean oil, liquid fermentation in bioreactor type Bioflo III. After 96 hours, the supernatant was separated from the cells by centrifugation and it was used as enzyme extract. The enzyme was immobilized by ionic gelation using sodium alginate and calcium chloride. For the encapsulation, a factorial design 22 was made with 2 and 0.5 mm atomizer sizes and CaCl2 (2 and 4% w/v) concentration. Subsequently, the encapsulate enzyme was characterized as to its encapsulation efficiency, stability, size and distribution size, and morphology
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Keywords


Lipase; Burkholderia Cepacia; Alginate; Encapsulation

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References


K. Jaeger, T. Eggert, Lipases for biotechnology. Cur. Opin. Biotec. 13 (2002)390-397.

F Hasan, A.A. Shah,., A.Hameed, Industrial applications of microbial lipases. Enz. and Micr. Techn. 39 (2006) 235-251.

Y. Shimada, Y.Watanabe, A. Sugihara, Y.Tominaga, Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing. J. Mol. Catalysis B: Enz. on ScienceDirect 17 (2002) 133-142.

G.S Padilha, G.L Castigioni, R M. Alegre, E.B. Tambourgi, Avaliação de diferentes íons na atividade da lipase de Pseudomonas cepacia. Exacta 7 (2010) 181-186.

R. Dalla-Vecchia, M.G. Nascimento, V.Soldi, Aplicações sintéticas de lipases imobilizadas em polímeros. Química Nova 27 (2004) 623-630.

A.H. King, Brow seawed extracts (Alginates). In: Food hydrocolloids (M.Glicksman, 1983).

D. Sebrão, V.D. Silva, M.G. Nascimento, M.A. Moreira, Imobilização de lipases em filme de caseinato de sódio/glicerol: aplicação na síntese de ésteres. Química Nova 30 (2007) 1182-1187.

G. M. Pastore, V. S. R Costa, M. G. B. koblitz, Purificação parcial e caracterização bioquímica de lipase extracelular produzida por nova linhagem de Rhizopus sp. Ciência e Tecnologia de Alimentos 23 (2003), 135-140.

G.A. Macedo, Y.K. Park, G.M. Pastore, Partial purification and characterization of an extracellular lipase from a newly isolated strain of Geotrichum sp. J. Braz. Soc. for Microb. 28 (1997), 90-95.

G.S. Padilha, Caracterização, purificação e encapsulamento de lipase de Burkholderia cepacia, Ph.D. dissertation, School of Chemical Engineering, UNICAMP, Campinas, SP, 2010.

M. Kordel, B. Hofmann, D.Schomburg, R. Schmid, Extracellular lipase of Burkholderia sp Strain ATCC 21808: purification, characterization, crystallization and preliminary X-ray diffraction data. J. Bacter. 177 (1991), 4836-4841.

W.A.M. Alloue, J. Destain, T. El-MEdjoub, H.Ghalfi, P. Kabran, P. Thonart, Comparison of Yarrowia lipolytica lipase immobilization yield of entrapment, adsorption, and covalent bond techniques. Appl. Bioch. Biot.150 (2008) p. 51-63.

H. Stamatis, A. Xenakis, Biocatalysis using microemulsion-based polymer gels containing lipase. J. Mol. Cat. B. 6 (1999) 399-406.

M.A.P. Cechinel, E. Skoronski, J.J.João, Avaliação da estabilidade de Lipozyme TL imobilizada em gel de gelatina aplicada na síntese de ésteres em meio orgânico. XVII Encontro de Química da Região Sul, FURG, 2009.

M. Rosenberg, S. Young, Whey proteins as microencapsulating agents. Microencapsulation of anhydrous milk fat-structure evaluation. Food Struc.12 (1993) 31-41.

K. Won, S., Kim, K.J. Kim, H.W. Park, S.J. Moon, Optimization of lipase entrapment in Ca-alginate gel beads. Proc. Bioch. 40 (2005), 2149–2154.

R. Mukai-Corrêa, A.S. Prata, I.D. Alvim, C.R.F. Grosso, Controlled release of protein from hydrocolloid gel microbeads before and after drying. Cur. Drug Del.1 (2004) 265-273.


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