Formation of Human ILPR G-Quadruplex in dsDNA

Soma Dhakal(1*), Zhongbo Yu(2), Ryan Konik(3), Deepak Koirala(4), Hanbin Mao(5)

(1) Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio 44242, USA., United States
(2) Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio 44242, USA., United States
(3) Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio 44242, USA., United States
(4) Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio 44242, USA., United States
(5) Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio 44242, USA., United States
(*) Corresponding author


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Abstract


The potential involvement of G-quadruplex in biological functions, such as gene expression and regulation, has been increased as G-quadruplex has been shown to exist in vivo. While the formation of G-quadruplex in ssDNA has been well studied, we show, using single-molecule and chemical footprinting, the formation of human ILPR G-quadruplex in dsDNA under physiological pH and K+ ion concentration. We also present the evidence that the ILPR G-quadruplex is mechanically stable with an unfolding force of 24 pN. Such a stable tetraplex structure in ILPR dsDNA is potential to control the transcription regulation of insulin gene
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Keywords


Human ILPR; G-Quadruplex; Single Molecule; DMS Footprinting

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References


M. C. Hammond-Kosack, B. Dobrinski, R. Lurz, K. Docherty, M. Kilpatrick, The Human Insulin Gene Linked Polymorphic Region Exhibits an Altered DNA Structure, Nucleic Acids Research, 20, (2), December 1992, 231-236.

P. Rotwein, S. Yokoyama, D. K. Didier, J. M. Chirgwin, Genetic Analysis of the Hypervariable Region Flanking the Human Insulin Gene, Am. J. Hum. Genet., 39, (3), September 1986, 291-299.

P. Catasti, X. Chen, R. K. Moyzis, E. M. Bradbury, G. Gupta, Structure-Function Correlations of the Insulin-Linked Polymorphic Region, J. Mol. Biol., 264, (3), December 1996, 534-545.

V. V. Jolad, F. K. Murad, J. R. P. Arnold, J. Fisher, Solution Conformation of D(C4ACAC4TGT)2; an Intramolecularly Folded i-Motif from the Insulin Minisatellite, Org. Biomol. Chem., 3, (12), May 2005, 2234-2236.

J. L. Huppert, S. Balasubramanian, G-Quadruplexes in Promoters Throughout the Human Genome, Nucleic Acids Res., 35, (2), December 2007, 406-413.

Z. Yu, J. D. Schonhoft, S. Dhakal, R. Bajracharya, R. Hegde, S. Basu, H. Mao, ILPR G-Quadruplexes Formed in Seconds Demonstrate High Mechanical Stabilities, J. Am. Chem. Soc., 131, (5), January 2009, 1876-1882

S. Dhakal, J. D. Schonhoft, D. Koirala, Z. Yu, S. Basu, H. Mao, Coexistence of an ILPR i-Motif and a Partially Folded Structure with Comparable Mechanical Stability Revealed at the Single-Molecule Level, J. Am. Chem. Soc., 132, (26), June 2010, 8991–8997.

K.-w. Zheng, D. Zhang, L.-x. Zhang, Y.-h. Hao, X. Zhou, Z. Tan, Dissecting the Strand Folding Orientation and Formation of G-Quadruplexes in Single- and Double-Stranded Nucleic Acids by Ligand-Induced Photocleavage Footprinting, J. Am. Chem. Soc., 133, (5), January 2011, 1475–1483.

M. I. Onyshchenko, T. I. Gaynutdinov, E. A. Englund, D. H. Appella, R. D. Neumann, I. G. Panyutin, Stabilization of G-Quadruplex in the Bcl2 Promoter Region in Double-Stranded DNA by Invading Short PNAs, Nucleic Acids Res, 37, (22), October 2009, 7570–7580.

D. Sun, L. H. Hurley, The Importance of Negative Superhelicity in Inducing the Formation of G-Quadruplex and I-Motif Structures in the C-Myc Promoter: Implications for Drug Targeting and Control of Gene Expression, J. Med. Chem., 52, (9), May 2009, 2863-2874.

P. S. Shirude, B. Okumus, L. Ying, T. Ha, S. Balasubramanian, Single-Molecule Conformational Analysis of G-Quadruplex Formation in the Promoter DNA Duplex of the Proto-Oncogene C-Kit, J. Am. Chem. Soc, 129, (24), May 2007, 7484-7485.

Y. Qin, E. M. Rezler, V. Gokhale, D. Sun, L. H. Hurley, Characterization of the G-Quadruplexes in the Duplex Nuclease Hypersensitive Element of the PDGF-A Promoter and Modulation of PDGF-A Promoter Activity by Tmpyp4, Nucleic Acids Res, 35, (22), November 2007, 7698–7713.D.


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