Structural and Functional Characterization of Thrombin Binding Aptamer Minor Loop
the author of the article can submit here a request for assignment of a DOI number to this resource!
Cost of the service: euros 10,00 (for a DOI)
The thrombin binding aptamer (29hTBA), identified with a SELEX approach, shows a significant affinity for the coagulation factor thrombin by recognizing the protein Exosite II (heparin binding site). The structure of 29hTBA consists of a G-quadruplex core flanked by two partially paired terminal strands. Literature data underline that the structure and stability of intramolecular G-quadruplexes can be profoundly influenced by the length and composition of the loops. Here, we investigated the role of the minor loop (10-11) composition by introducing a single A-T mutation at position 11. A comparative structural investigation of the wild type and the mutant aptamers evidenced that this loop is not largely impairing the folding of the G-quadruplex core. Additionally, thrombin binding analysis suggested that this loop is not directly involved in protein binding at exosite II
Copyright © 2011 Praise Worthy Prize - All rights reserved.
B. Gatto, M. Palumbo, C. Sissi, Nucleic Acid Aptamers Based on the G-Quadruplex Structure: Therapeutic and Diagnostic Potential, Current Medicinal Chemistry, Volume 16, 2009, Pages 1248-1265.
L. Bock, L. Griffin, J. Latham, E. Vermaas, J. Toole, Selection of single-stranded DNA molecules that bind and inhibit human Thrombin, Nature, Volume 355, 1992, Pages 564-566.
D.M. Tasset, M.F. Kubik, W. Steiner, Oligonucleotide inhibitors of human thrombin that bind distinct epitopes, J. Mol. Biol., Volume 272, 1997, Pages 688-698.
R.F. Macaya, P.Schultze, F.W. Smith, J.A. Roe, J. Feigon, Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution, Proc. Natl. Acad. Sci. U. S. A., Volume 90, 1993, Pages 3745-3749.
I. Smirnov, R.H. Shafer, Effect of loop sequence and size on DNA aptamer stability, Biochemistry, Volume 39, 2000, Pages 1462-1468.
F., Macaya, Structural and functional characterization of potent antithrombotic oligonucleotides possessing both quadruplex and duplex motif, Biochemistry, Volume 34, 1995, Pages 4478-4492.
S. Niedle, S. Balasubramanian, Quadruplex nucleic acids (The Royal Society of Chemistry, 2006, Pages 34-35).
J. Kypr, M. Vorlickova, Circular dichroism and conformational polymorphism of DNA, Nucleic Acid Research, Volume 37, 2009, Pages 1713-1725.
S. Nagatoishi, Y. Tanaka, K. Tsumoto, Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions, Biochem. Biophys. Res. Commun., Volume 352, 2007, Pages 352, 812-7.
M. Tracy, T.M. Bryan, M.B. Jarstfer, Interrogation of G-quadruplex–protein interactions, Methods, Volume 43, 2007, Pages 332–339.
- There are currently no refbacks.
Please send any question about this web site to firstname.lastname@example.org
Copyright © 2005-2019 Praise Worthy Prize