Template Assembled Synthetic G-Quadruplex: a Novel Biomolecular System for Investigating the Interactions of Ligands with Constrained Quadruplex Conformation

R. Bonnet; P. Murat; N. Spinelli; A. Van Der Heyden; P. Labbé; P. Dumy; E. Defrancq

doi:10.15866/irebic.v2i3.1515

Template Assembled Synthetic G-Quadruplex: a Novel Biomolecular System for Investigating the Interactions of Ligands with Constrained Quadruplex Conformation

R. Bonnet^(1*), P. Murat⁽²⁾, N. Spinelli⁽³⁾, A. Van Der Heyden⁽⁴⁾, P. Labbé⁽⁵⁾, P. Dumy⁽⁶⁾, E. Defrancq⁽⁷⁾

^(*) Corresponding author

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Abstract

We report on the design of a new biomolecular device based on the concept of Template Assembled Synthetic G-Quadruplex (TASQ), where quadruplex DNA structures are assembled on a scaffold for constraining G-quadruplex conformation. As a proof of concept, parallel-stranded conformation of G-quadruplex was prepared by using a cyclodecapeptide as the template. As anticipated the use of the scaffold allows the precise control of the conformation of the quadruplex and increases dramatically the stability of the motif. This device was used for investigating by Surface Plasmon Resonance the affinity of ligands for G-quadruplex DNA
Copyright © 2011 Praise Worthy Prize - All rights reserved.

Keywords

Click-chemistry; G-quadruplex; Surface Plasmon Resonance; Template

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