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Preparation and AFM-Characterization of Self-Assembled Monolayers Functionalized with a Thrombin Binding Aptamer
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Abstract
Self-assembled monolayers on silicon oxide surfaces functionalized with a unimolecular DNA G-quadruplex derived from the thrombin binding aptamer have been prepared. The formation of the G-quadruplex in the presence of potassium ions is observed by atomic force microscopy (AFM). The results presented here are interesting for the fabrication of nanostructured systems for sensing applications
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References
E. Engel, A. Michiardi, M. Navarro, D. Lacroix, J. A. Planell, Nanotechnology in regenerative medicine: the materials side. Trends in Biotechnology, Volume 26 (Issue 1), January 2008, Pages 39-47.
A. Sassolas, D. Leca-Bouvier, L. J. Blum, DNA biosensors and microarrays. Chemical Reviews, Volume 108, (Issue 1), January 2008, Pages 109-139.
J. Liu, Z. Cao, Y. Lu, Functional nucleic acids sensors. Chemical Reviews, Volume 109, (Issue 5), 2009, Pages 1948-1998.
E. N. Brody, L. Gold, Aptamers as therapeutic and diagnostic agents, Reviews in Molecular Biotechnology, Volume 74, (Issue 1), March 2000, Pages 5-13.
L. C. Bock, L. C. Griffin, J. A. Latham, E. H. Vermaas, J. J. Toole, Selection of single-stranded DNA molecules that bind and inhibit human thrombin, Nature, Volume 355, (Issue 6360), February 1992, Pages 564-566.
R. F. Macaya, P. Schultze, F. W. Smith, J. A. Roe, J. Feigon, Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution, Proceedings of the National Academy of Sciences USA, Volume 90, (Issue 8), April 1993, Pages 3745-3749.
T. Mairal, V. C. Ozalp, P. Lozano Sanchez, M. Mir, I. Katakis, C. K. O'Sullivan. Aptamers: molecular tools for analytical applications, Analytical Bioanalytical Chemistry, Volume 390, (Issue 4), February 2008, Pages 989-1007.
S. Nagatoishi, T. Nojima, E. Galezowska, B. Juskowiak, S. Takenaka, G quadruplex-based FRET probes with the thrombin-binding aptamer (TBA) sequence designed for the efficient fluorometric detection of the potassium ion, Chembiochem, Volume 7, (Issue 11), November 2006, Pages 1730-1737.
M. Tintoré, A. Aviñó, F.M. Ruiz, R. Eritja, C. Fàbrega, Development of a novel fluorescence assay based on the use of the thrombin-binding aptamer for the detection of O6-alkylguanine-DNA alkyltransferase activity, Journal of Nucleic Acids, Volume 2010, Article ID 632041, September 2010, 9 Pages, doi: 10.4061/2010/632041.
B. Manning, S. J. Leigh, R. Ramos, J. Preece, R. Eritja, Fabrication of patterned surfaces by photolithographic exposure of DNA-hairpins carrying a novel photolabile group. Journal of Experimental Nanoscience, Volume 5, (Issue 1), January 2010, Pages 26-39.
I. Smirnov, R. H. Shafer, Effect of loop sequence and size on DNA aptamer stability. Biochemistry, Volume 39, 2000, Pages 1462-1468.
V. C. Diculescu, A. M. Chiorcea-Paquim, R. Eritja, A. M. Oliveira-Brett, Thrombin binding aptamer quadruplex formation: AFM and voltammetric characterization, Journal of Nucleic Acids, September 2010, Article ID 841932, 8 pages, doi:10.4061/2010/841932.
V. C. Diculescu, A. M. Chiorcea-Paquim, R. Eritja, A. M. Oliveira-Brett, Evaluation of the structure-activity relationship of thrombin binding aptamers by voltammetry and atomic force microscopy, Journal of Electroanalytical Chemistry, Volume 656, (Issue 1-2), June 2011, Pages 159-166.
S. Diegoli, P. M. Mendes et al., pH-dependent gold nanoparticles self-organization on functionalized Si/SiO2 surfaces, Journal of Experimental Nanoscience, Volume 1, (Issue 3), 2006, Pages 333-353.
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