Preparation and AFM-Characterization of Self-Assembled Monolayers Functionalized with a Thrombin Binding Aptamer

Brendan Manning(1*), Isaac Gállego(2), María Tintoré(3), Mª Carme Fàbrega(4), Anna Aviñó(5), Ramon Eritja(6)

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(*) Corresponding author

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Self-assembled monolayers on silicon oxide surfaces functionalized with a unimolecular DNA G-quadruplex derived from the thrombin binding aptamer have been prepared. The formation of the G-quadruplex in the presence of potassium ions is observed by atomic force microscopy (AFM). The results presented here are interesting for the fabrication of nanostructured systems for sensing applications
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G-quadruplex; Self-assembled Monolayers; DNA; Thrombin Binding Aptamer; AFM

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