Thermodynamic Properties and Electron Transfer of the Interaction Between Pyropheophorbide a Methyl Ester and Copper: the Nature of Binding Forces

S. Al-Omari(1*)

(1) Department of Physics, Faculty of Science, The Hashemite University, Zarqa 13115, Jordan., Jordan
(*) Corresponding author

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Pyropheophorbide methyl ester (PPME) is clinically used as antitumor drug. Understanding of the strong interaction between PPME and Cu2+ could contribute to understand its pharmacodynamics and pharmacokinetics. The interaction between PPME and Cu2+ was investigated using fluorescence and UV-vis techniques. The binding constants of Cu2+ with PPME were determined at different temperatures depending on the fluorescence quenching results. Furthermore, the thermodynamic functions of standard enthalpy (ΔH0) and standard entropy (ΔS0) for the binding reaction were determined according to the van’t Hoff equation, which indicated that electron transfer, electrostatic, and hydrophobic interactions are important driving forces for PPME-Cu2+ association
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Electron Transfer; Thermodynamics; Electronic Absorption; Exciplex Formation; Electrostatic Interaction; Cancer Angiogenesis

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C. Kanony, A. S. Fabiano, J. L. Ravanat, P. Vicendo, N. Paillous, Photosensitization of DNA Damage by a New Cationic Pyropheophorbide Derivative: Sequence-specific Formation of a Frank Scission, Photochem. Photobiol 77 (2003), 659-667.

S. Al-Omari, A. Ali, Photodynamic Activity of Pyropheophorbide Methyl Ester and Pyropheophorbide a in Dimethylformamide Solution, Gen. Physiol. Biophys 28 (2009), 70-77.

G. J. Brewer, Copper Control as an Antiangiogenic Anticancer Therapy: Lessons From Treating Wilson's Disease. Exp. Biol. Med 226 (2001), 665-673.

S. Brem, Angiogenesis and Cancer Control: from Concept to Therapeutic Trial, Cancer. Control 6 (1999), 436-458.

E. J. Margalioth, J. G. Schenker, M. Chevion, Copper and Zinc Levels in Normal and Malignant Tissues, Cancer 52 (1983), 868-872.

S. Al-Omari, M. Al-Noaimi, Spectroscopic Analysis of the Binding Interaction of Cationic Cu2+ With Pyropheophorbide-a Me Ester: Targeting Tumor in a Copper-Dependent Manner, Int. Rev. Phys. 4 (2010) 152-160

L. Lawniczak, J. Douch, P. Behra, Optimization of the Analytical Detection of EDTA by in Natural Waters, J. Anal. Chem. 364 (1999) 727-731.

X. Zhang, Spectral Study of the Interaction Between Chelerythrine Chloride and Adenosine, Spec. Lett 40 (2007) 615-626.

C. Chen, M. Ma, J. Zhang, L. Wang, B. Xiang, Spectroscopic Investigation of the Interaction of Bovine Serum Albumin with a Novel Cardiac Agent V-09, Spectroscopy 22 (2008) 43-50.

G. Neme´thy, H. A. Scheraga, The Structure of Water and Hydrophobic Bonding in Proteins. I. A Model for the Thermodynamic Properties of Liquid Water, J. Phys. Chem. 66 (1962) 1773-1789.

X. Z. Feng, X. Jinr, Y. Qu., X. W. He, Studies on Ion Effect on the Binding Interaction Between HP and BSA, Chem. J. Chinese Univ. 17 (1996) 866-869.

S. N. Tiamseff, Proteins of Biological Fluids, (Pergamon Press, Oxford, 1972).

P. D. Ross, S. Subramanian, Thermodynamics of Protein Association Reaction: Forces Contribute on to Stability, Biochemistry 20 (1981) 3096-3102.

S. A. Moore, K. M. Glenn, R. Palepu, Fluorescence Quenching of Safranine T by Inorganic Anions in Tween Micelles, Can. J. Chem. 83 (2005) 2067-2072.

B. Valeur, Molecular Fluorescence: Principles and Applications, (Wiley-VCH Verlag GmbH, 2001)

R. A. Marcus, On the Theory of Oxidation-Reduction Reactions Involving Electron Transfer. II. Applications to Data on the Rates of Isotopic Exchange Reactions, J. Chem. Phys. 26 (1957) 867.

C. Sato, K. Kikuchi, K. Okamura, Y. Takahashi, T. Miyashi, New Aspects on Fluorescence Quenching by Molecular Oxygen. 2. Inhibition of Long-Distance Electron Transfer in Acetonitrile, J. Phys. Chem. 99 (1995) 16925-16931.

I. R. Gould, D. Ege, J. E. Moser, S, Farid, Efficiencies of Photoinduced Electron-Transfer Reactions: Role of the Marcus Inverted Region in Return Electron Transfer Within Geminate Radical-Ion Pairs, J. Am. Chem. Soc. 112 (1990) 4290.

J. R. Lakowics, Principles of Fluorescence Spectroscopy, (1st edn, Plenum Press, New York, 1983).

J. E. Turner, Atoms, Radiation, and Radiation Protection, (2nd ed John Wiley&Sons, Inc, 1995).

Arguslab. ArgusLab (tm). Program package. Version 4.0. tware LLC.

L. Stryer, Fluorescence energy transfer as a spectroscopic ruler, Annu. Rev. Biochem. 47 (1978), 819-846.


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