Multivariate and T1 Analysis on Serum Samples from Coronary Heart Disease Patients (CHD): a Pilot Study


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Abstract


The one dimensional 1H  NMR  at 480C of serum samples were acquired for CHD (coronary heart disease patients) (n =26). The reduced ultra binned spectra region 0.77-0.81ppm of 0.01ppm at 480C comprising of methyl protons of VLDL, LDL and HDL were found to provide discriminating clusters in the Principal Component Analysis score plots when compared with healthy control serum samples. The spin lattice relaxation times (T1) of alanine, lactate and methyline protons of lipoproteins were found to be significantly reduced in univariate analysis when compared with control samples. The T1 values of these metabolites were further subjected to Discriminant Function Analysis (DFA) for control and CHD patients. It was observed that 84.6% of correct classification was achieved. The analysis demonstrated that NMR can be an effective method for diagnosis of CHD in clinical setup
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Keywords


Serum; Metabolites; Lipoproteins; Spin Lattice Relaxation Time

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