Dye-Liposome Interactions: Dye Localization in Neutral Lipid Bilayers


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Abstract


A large number peptides and proteins can interact with lipid membranes, these contacts serve different functions and their deregulation is associated to different human disorders. Although cyanine dyes have become standard optical probes to study protein conformational changes upon membrane binding, their interactions with lipid bilayers remain essentially unexplored. In the present work we characterize the association of Congo Red (CGR), merocyanine (MER) and pinacyanol (PIN) with DMPC liposomes by spectrophotometry. These dyes exhibit different association constants and localization at the liposome membrane. Dye affinity to liposome exhibits the following gradation: MER>PIN>CGR. Importantly, bound MER and PIN are localized in inner nonpolar region of the DMPC bilayer whereas CGR interacts preferentially with the more polar bilayer surface. These dye properties should be taken into account when dissecting protein-membrane interactions using spectroscopic approaches
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Keywords


Association Constant; Congo Red; Dimerization Constant; Dye; Liposome; Merocyanine; Pinacyanol

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