Ag+-Induced Arrangement of Poly(dC) into Compact Ring-Shaped Structures

(*) Corresponding author

Authors' affiliations

DOI's assignment:
the author of the article can submit here a request for assignment of a DOI number to this resource!
Cost of the service: euros 10,00 (for a DOI)


We report the synthesis, circular dichroism (CD) and atomic force microscopy (AFM) characterization of DNA-based ring-shaped nanostructures resulting from folding of 700 base poly(dC) strands in the presence of silver ions (Ag+). The AFM contour length and height analyses of the molecules suggest that the poly(dC) strand in complex with Ag+ can wrap around itself forming a double helical circular molecule. Incubation of stoichiometrical complex between poly(dG)-poly(dC) with Ag+ at 50°C leads to dissociation of the strands and to spontaneous folding of the G-strand into monomolecular G-quadruplex and the C-strand into double stranded C-C duplex stabilized by Ag-ions
Copyright © 2010 Praise Worthy Prize - All rights reserved.


AFM; CD; DNA; Poly(dC); G-quadruplex; Silver Ions

Full Text:



Arraye Arya, S.K. and Yang, J.T. Optical rotatory dispersion and circular dichroism of silver(I):Polyribonucleotide complexes. Biopolymers, Volume 14 ,(Issue 9), 1975, Pages 1847-1861.

Dattagupta, N. and Crothers, D.M. Solution structural studies of the Ag(I)-DNA complex. Nucleic Acids Res, Volume 9, (Issue 12), 1981, Pages 2971-2985.

Daune, M., Dekker, C.A. and Schachman, H.K. Complexes of silver ion with natural and synthetic polynucleotides. Biopolymers, Volume4, (Issue 1), 1966 ,Pages 51-76.

Dove, W.F. and Davidson, N. Cation effects on the denaturation of DNA. Journal of Molecular Biology, Volume5, (Issue 5), 1962, Pages 467-478.

Eichhorn, G.L., Butzow, J.J., Clark, P. and Tarien E. Interaction of metal ions with polynucleotides and related compounds. X. Studies on the reaction of silver(I) with the nucleosides and polynucleotides, and the effect of silver(I) on the zinc(II) degradation of polynucleotides. Biopolymers, Volume 5, (Issue 3), 1967, Pages 283-296.

Izatt, R.M., Christensen, J.J. and Rytting, J.H. Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and and nucleotides. Chemical Reviews, Volume 71, (Issue 5), 1971, Pages 439-481.

E Jensen, R.H. and Davidson, N. Spectrophotometric, potentiometric, and density gradient ultracentrifugation studies of the binding of silver ion by DNA. Biopolymers, Volume 4, (Issue 1), 1966, Pages 17-32.

Shin, Y.A. and Eichhorn, G.L. Induction of helicity in polyuridylic acid and polyinosinic acid by silver ions. Biopolymers, Volume 19, (Issue 3), 1980, Pages 539-556.

Yamane, T. and Davidson, N. (1962) Note on the spectra of the mercury(II) and silver(I) complexes of some polyribonucleotides and ribonucleic acid. Biochim. Biophys. Acta, Volume55, 1960, Pages 780-782.

Yamane, T. and Davidson, N. On the complexing of deoxyribonucleic acid by silver(I). Biochim. Biophys. Acta, 55, 1962, Pages 609-621.

Eichhorn, G.L. Inorganic Biochemistry. G. L. Eichhorn, (ed. Eisevier, Amsterdam, New York, 1973, Vollume II, Chapter 33).

Shukla, S. and Sastry, M. Probing differential Ag+-nucleobase interactions with isothermal titration calorimetry (ITC). Towards patterned DNA metallization Nanoscale, Volume 1, 2009, Pages 122-127.

Kistenmacher, T.J., Rossi, M. and Marzilli, L.G. Crystal and molecular structure of (nitrato)(1-methylcytosine)silver(I): an unusual crosslinked polymer containing a heavy metal and a modified nucleic acid constituent. Inorganic Chemistry, Volume 18,(Issue 2), 1979, Pages 240-244.

Ritchie, C.M., Johnsen, K.R., Kiser, J.R., Antoku, Y., Dickson, R.M. and Petty, J.T. Ag Nanocluster Formation Using a Cytosine Oligonucleotide Template. The Journal of Physical Chemistry C, Volume 111, (Issue 1), 2006 Pages 175-181.

Ono A, Cao S, Togashi H, Tashiro M, Fujimoto T, Machinami T, Oda S, Miyake Y, Okamoto I, Tanaka Y. Specific interactions between silver(I) ions and cytosine-cytosine pairs in DNA duplexes. Chem Commun (Camb).(Issue 39) 2008, Pages 4825482-7.

Kotlyar, A.B., Borovok, N., Molotsky, T., Fadeev, L. and Gozin, M. In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I. Nucleic Acids Res, Volume 33, (Issue 2), 2005, Pages 525-535.

Cantor, C.R., Warshaw, M.M. and Shapiro, H. Oligonucleotide interactions. 3. Circular dichroism studies of the conformation of deoxyoligonucleotides. Biopolymers, Volume 9, (Issue 9), 1970, Pages 1059-1077.

Wells, R.D., Larson, J.E., Grant, R.C., Shortle, B.E. and Cantor, C.R. Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences. Journal of Molecular Biology, Volume54, (Issue 3), 1970, Pages 465-497.

Horcas, I., Fernandez, R., Gomez-Rodriguez, J.M., Colchero, J., Gomez-Herrero, J. and Baro, A.M. WSXM: A software for scanning probe microscopy and a tool for nanotechnology. Rev Sci Instrum, Volume78, (Issue 1), 2007, Pages 013705.

Borovok, N., Molotsky, T., Ghabboun, J., Porath, D. and Kotlyar, A. Efficient procedure of preparation and properties of long uniform G4-DNA nanowires. Anal Biochem, Volume 374, (Issue 1), 2008, Pages 71-78.

Petty, J.T., Zheng, J., Hud, N.V. and Dickson, R.M. DNA-Templated Ag Nanocluster Formation. Journal of the American Chemical Society, Volume 126, (Issue 6), 2004, Pages 5207-5212.

Lubitz, I., Zikich, D. and Kotlyar, A.B. Specific High-Affinity Binding of Thiazole Orange to Triplex and G-Quadruplex DNA. Biochemistry, Volume 49 (Issue 17),2010, Pages 3567–3574.

D. Gorinevsky, S. Boyd, G. Stein, Optimization-based tuning of low-bandwidth control in spatially distributed systems, American Control Conference, Vol. 3, pp. 2658–2663, Denver, CO, June 2003.


  • There are currently no refbacks.

Please send any question about this web site to
Copyright © 2005-2023 Praise Worthy Prize