Ag+-Induced Arrangement of Poly(dC) into Compact Ring-Shaped Structures

Dragoslav Zikich(1), Irit Lubitz(2), Alexander Kotlyar(3*)

(1) Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel
(2) Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel
(3) Department of Biochemistry, George S. Wise Faculty of Life Sciences, The Center for Nanoscience and Nanotechnology, Tel Aviv University, Israel
(*) Corresponding author


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Abstract


We report the synthesis, circular dichroism (CD) and atomic force microscopy (AFM) characterization of DNA-based ring-shaped nanostructures resulting from folding of 700 base poly(dC) strands in the presence of silver ions (Ag+). The AFM contour length and height analyses of the molecules suggest that the poly(dC) strand in complex with Ag+ can wrap around itself forming a double helical circular molecule. Incubation of stoichiometrical complex between poly(dG)-poly(dC) with Ag+ at 50°C leads to dissociation of the strands and to spontaneous folding of the G-strand into monomolecular G-quadruplex and the C-strand into double stranded C-C duplex stabilized by Ag-ions
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Keywords


AFM; CD; DNA; Poly(dC); G-quadruplex; Silver Ions

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References


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