Enhanced Lycopene Recovery from Tomato Processing Waste by Enzymatic Degradation of Plant Tissue Components

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Eight commercial enzyme preparations with pectinolytic, cellulolytic, hemicellulolytic and proteolytic activities were tested for their ability to enhance lycopene extraction from tomato peels. Screening experiments were performed at 40 °C by subjecting the peels to a 1-h enzyme incubation followed by 1-h hexane extraction. The resulting yields were between 51 and 195.9 mg of lycopene per 100 g of dry tomato peels, while the value obtained for the untreated peels was 23.7 mg /100 g. Synergistic and antagonist effects were observed when different enzyme products were used in 50:50 (v/v) combination. The preparations richest in cellulase and pectinase were the most efficient, with an up to 10-fold increase in extraction yield. A statistical analysis of factors affecting lycopene extraction revealed that enzyme dosage was the most influential, followed by temperature and enzyme incubation time. A strong interaction was also found between the latter two variables
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Bioprocessing; Cell Wall Polysaccharides; Enzymes; Lycopene; Plant Tissue; Tomato Processing Waste

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I.S. Arvanitoyannis, T.H. Varzakas, Vegetable waste treatment: comparison and critical presentation of methodologies, Crit. Rev. Food Sci. Nutr. 48 (2008) 205–247.

A. Schieber, F.C. Stintzing, A. Carle, By-products of plant food processing as a source of functional compounds – recent developments, Trends Food Sci. Technol. 12 (2001) 401–413.

N. Balasundram, K. Sundram, S. Samman, Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses, Food Chem. 99 (2006) 191–203.

G. Laufenberg, B. Kunz, M. Nystroem, Transformation of vegetable waste into value added products: (A) the upgrading concept; (B) practical implementations, Bioresource Technol. 87 (2003) 167–198.

WPTC, World production estimate as of 1 June 2008, Release # 18, http://www.wptc.to/releases/releases18.pdf (accessed March 10, 2009).

J. Shi, M. Le Maguer, G. Mazza, G. Functional foods (CRC Press, 2002, pp. 135–167).

P. Di Mascio, S. Kaiser, H. Sies, Lycopene as the most efficient biological carotenoid singlet oxygen quencher, Arch. Biochem. Biophys. 274 (1989) 532–538.

A.O. Omoni, R.E. Aluko, The anti-carcinogenic and anti-atherogenic effects of lycopene: a review, Trends Food Sci. Technol. 16 (2005) 344–350.

E. Giovannucci, Tomato products, lycopene, and prostate cancer: a review of the epidemiological literature, J. Nutr. 35 (2005) 2030S–2031S.

S.K. Sharma, M. Le Maguer, Lycopene in tomatoes and tomato pulp fractions, Ital. J. Food Sci. 8 (1966) 107–113.

E. Sabio, M. Lozano, V. Montero de Espinosa, R.L. Mendes, A.P. Pereira, A.F. Palavra, J.A. Coelho, Lycopene and β-carotene extraction from tomato processing waste using supercritical CO2, Ind. Eng. Chem. Res. 42 (2003) 6641–6646.

N.L. Rozzi, R.K. Singh, R.A. Vierling, B.A. Watkins, Supercritical fluid extraction of lycopene from tomato processing byproducts, J. Agric. Food Chem. 50 (2002) 2638–2643.

W.M. Harris, A.R. Spurr, Chromoplasts of tomato fruits. II. The red tomato, Am. J. Bot. 56 (1969) 380–389.

S. Xianquan, J. Shi, Y. Kakuda, J. Yueming, Stability of lycopene during food processing and storage, J. Med. Food 8 (2005) 413–422.

H. Dominguez, M.J. Nunez, J.M. Lema, Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds: a review, Food Chem. 49 (1994) 271–286.

P. Hanmoungjai, D.L. Pyle, K. Niranjan, Enzyme-assisted water-extraction of oil and protein from rice bran, J. Chem. Technol. Biotechnol. 77 (2002) 771–776.

A.B. Bautista-Ortín, A. Martínez-Cutillas, J.M. Ros-Garcia, J.M. López-Roca, E. Gómez-Plaza, Improving colour extraction and stability in red wines: the use of maceration enzymes and enological tannins, Int. J. Food Sci. Technol. 40 (2005) 867–878.

R.I. Santamaria, M.D. Reyes-Duarte, E. Barzana, D. Fernando, F.M. Gama, M. Mota, A. López-Munguía, Selective enzyme-mediated extraction of capsaicinoids and carotenoids from Chili Guajillo Puya (Capsicum annuum L.) using ethanol as solvent, J. Agric. Food Chem. 48 (2000) 3063–3067.

E. Barzana, D. Rubio, R.I. Santamaria, O. Garcia-Correa, F. Garcia, V.E. Ridaura Sanz, A. López-Munguía, Enzyme-mediated solvent extraction of carotenoids from marigold flowers (Tagetes erecta), J. Agric. Food Chem. 50 (2002) 4491–4496.

S.M. Choudhari, L. Ananthanarayan, Enzyme aided extraction of lycopene from tomato tissue, Food Chem. 102 (2007) 77–81.

Merck & Co., The Merck Index 11th edn. (Rahway, 1989).

W.W. Fish, P. Perkins-Veazie, J.K. Collins, A quantitative assay for lycopene that utilizes reduced volumes of organic solvents, J. Food Comp. Anal. 15 (2002) 309–317.

A. Zuorro, Estrazione del licopene dai residui di lavorazione dell’industria del pomodoro, M.S. dissertation, Dept. Chem. Eng., Sapienza Univ., Rome, Italy, 2005.

K.C. Gross, Fractionation and partial characterization of cell walls from normal and non-ripening mutant tomato fruit, Physiol. Plantarum 62 (1984) 25–32.

M.C. McCann, M. Bush, D. Milioni, P. Sado, N.J. Stacey, G. Catchpole, M. Defernez, N.C. Carpita, H. Hofte, P. Ulvskov, R.H. Wilson, K. Roberts, Approaches to understanding the functional architecture of the plant cell wall, Phytochemistry 57 (2001) 811–821.

C. Somerville, S. Bauer, G. Brininstool, M. Facette, T. Hamann, J. Milne, E. Osborne, A. Paredez, S. Persson, T. Raab, S. Vorwek, H. Youngs, Towards a systems approach to understanding plant cell walls, Science 306 (2004) 2206–2211.

J.M. Ribeiro, C.S. Pereira, N.C. Soares, A.M. Vieira, J.A. Feijo, P.A. Jackson, The contribution of extensin network formation to rapid, hydrogen peroxide-mediated increases in grapevine callus wall resistance to fungal lytic enzymes, J. Exp. Bot. 57 (2006) 2025–2035.

K. Sriroth, R. Chollakup, S. Chotineerana, K. Piyachomkwan, C.G. Oates, Processing of cassava waste for improved biomass utilization, Bioresource Technol. 71 (2000) 63–69.

R. Bagger-Jørgensen, A.S. Meyer, Effects of different pre-press maceration treatments on the release of phenols into blackcurrant juice, Eur. Food Res.Technol. 219 (2004) 620–629.

E. Chiacchierini, G. Mele, D. Restuccia, G. Vinci, Impact evaluation of innovative and sustainable extraction technologies on olive oil quality, Trends Food Sci. Technol. 18 (2007) 299–305.

I. Çinar, Effects of cellulase and pectinase concentrations on the colour yield of enzyme extracted plant carotenoids, Process Biochem. 40 (2005) 945–949.

M.A. Ha, D.C. Apperley, M.C. Jarvis, Molecular rigidity in dry and hydrated onion cell walls, Plant Physiol. 115 (1997) 593–598.

M. Norsker, L. Bloch, J. Adler-Nissen, Enzymatic degradation of plant cell wall polysaccharides: the kinetic effect of competitive adsorption, Nahrung 43 (1999) 307–310.

G. A. Lewis, D. Mathieu, R. Phan-Tan-Luu, Pharmaceutical Experimental Design (Marcel Dekker, 1999).

C. Leoni, Focus on lycopene, Acta Horticulturae 613 (2003) 357–363.

C.C. Caris-Veyrat, A. Schmid, M. Carail, V. Böhm, Cleavage products of lycopene produced by in vitro oxidations: characterization and mechanisms of formation, J. Agric. Food Chem. 51 (2003) 7318–7325.


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