Optimization of Cassava Starch Hydrolysis by Malt Extract, α-Amylase and Glucoamylase
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Cassava starch hydrolysis was investigated in this study using malt extract, alpha amylase and glucoamylase. The effects of process variables, namely: temperature, pH and time were studied and optimized for hydrolysis of cassava (Manihot esculenta) flour to glucose syrup. Three levels of process variables were used for the study. The three levels of process variables were: temperature (60, 67 and 74 oC), time (1.5, 2.0 and 2.5 h) and pH (4.5, 5 and 5.5). A polynomial regression model was developed using the experimental data. The results showed that production of reducing sugar was strongly affected by the variation of variables on malt extract, alpha amylase and glucoamylase hydrolysis of cassava starch. The fit of the model was expressed by the coefficient of determination R2 which was found to be 0.987 indicating that 98.7 % of the variability in the response can be explained by the model. The value also indicates that only 1.3% of the total variation is not explained by the model. This shows that equation (2) is a suitable model to describe the response of the experiment pertaining to reducing sugar production. The statistical significance of the model was validated by F-test for analysis of variance (p ≤ 0.05). For malt extract, alpha amylase and glucoamylase hydrolysis, the optimum value of temperature, time and pH were found to be 74 oC; pH 5.5 and time 1.5 h. The maximum reducing sugar production at optimum condition was 268 g/l representing 76.57 % conversion or 76.57 dextrose equivalent (DE)
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Guzman-Maldonado H, Paredes-Lopez O. Amylolytic enzymes and products derived from starch: a review. Crit Rev Food Sci Nutr., 1995, 35:373–403.
Crabb WS, Shetty JK. Commodity scale production of sugars from starches. Curr Opin Microbiol., 1999, 2:252–6.
Giordano RL, Hirano PC, Goncalves LR, Netto WS. Study of biocatalyst to produce ethanol from starch. Coimmobilization of glucoamylase and yeast in gel. Appl Biochem Biotechnol., 2000; 86: 643–54.
Glazer AH, Nikaido H. Microbial biotechnology: fundamentals of applied microbiology. New York: WH Freeman & Co., 1994, p. 365.
Reeve, A., Starch hydrolysis : processes and equipment. In Starch Hydrolysis Products. W orldwide T echnology, oduction and Applications, eds F. W. Schenck & R. E. Hebeda. VCH Publishers, New York, 1992, pp. 79È120.
Dziedzic, S. Z. & Kearsley, M. W., Physico-chemical properties of glucose syrups. In Glucose Syrups: Science and T echnology, eds S. Z. Dziedzic & M. W. Kearsley. Elsevier Applied Science Publishers, London, 1984, pp. 137È68.
Fullbrook, P. D., The enzymic production of glucose syrup. In Glucose Syrups: Science and T echnology, eds S. Z. Dziedzic & M. W. Kearsley. Elsevier Applied Science Publishers, London, 1984, pp. 65È114.
Howling, D., Glucose syrup: production, properties and applications. In Starch Hydrolysis Products. W orldwide T echnology, Production and Applications, eds F. W.Schenck & R. E. Hebeda. VCH Publishers, New York, 1992, pp. 277È317.
Okada, M. & Nakakuki, T., Oligosaccharides : production, properties and applications. In Starch Hydrolysis Products. W orldwide T echnology, Production and Applications, eds F. W. Schenck & R. E. Hebeda. VCH Publishers, New York, 1992, pp. 335È66.
J. De Clerck, in Cours de Brasserie, Universitt de Louvain, Ed. (Institut gronomique, Belgique, 1962), pp. 410-421
C. JSM Silva, G. G. Ubitz, and C. P. Artur, “Optimisation of a serine protease coupling to Eudragit S-100 by experimental design techniques”, J. Chem. Technol. Biotech., 2006, vol. 81, pp. 8-16.
Ilori, M. O., Akingbala, J. O., Oguntimehin, G. B. and Ogundiwin, J. O. Effect of grain bed thickness, duration of steeping and germination on the malting properties of improved Nigerian Sorghum varieties. Lebensmittel–wissen schaft und – Technologie., 1990, 23, pp. 505-512.
Solomon, B. O., Layokun, S. K., Idowu, A. O. and Ilori, M. O. Prospects for the utilization of endogenous enzymes in Sorghum Malt in the hydrolysis of starch for ethanol production: Case study with Breadfruit flour. Food Biotechnol., 1994, 8 (2&3) pp.243-255.
Briggs, A.W., Wadeson, A.,Stahan, R. and Taylor, J. F. The use of extruded barley, wheat and maize as adjunct in mashing. Journal of the institute of Brewing, 1986, 92, pp. 468-474.
Aderibigbe, F.A. A Comparative Study On Hydrolysis Of Cassava Starch Using Sorghum Malt Extract, Acid and Enzyme-Enzyme Media. Ph.D. Thesis (unpublished), Obafemi Awolowo University, Ile-Ife, Nigeria, 2011.
A. F. Aderibigbe; A. N. Anozie, L. A. Adejumo and R. U. Owolabi, Optimization of Cassava Starch Hydrolysis by sorghum Malt. Journal of New Clues in Sciences 2(2012) 50-58.
Miller, G. L. Use of dinitro-salicylic acid reagent for the determination of reducing sugar, Anal. Chem., 1959, 31 (3) pp. 426-428.
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