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Effect of Cross-Linking Agents on Cytocompatibility of a New Reline Acrylic Resin
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Abstract
This study determined the cytotoxicity in vitro of two experimental autopolymerizing reline systems highly cross-linked and one commercial reline resin. Sample disks of the three hard reline acrylic resins were fabricated under aseptic conditions. L929 cells were cultured in 96-well plates and incubated for 24 h in Eagle's medium. Eluates were prepared by placing three samples into sterile glass vial with 9 mL of Eagle's medium and incubating at 37ºC for 24 h. The cytotoxic effect from the eluates was evaluated using the 3H-thymidine incorporation and MTT assays, which reflect DNA synthesis levels and cell metabolism, respectively. The data were statistically analyzed one-way analyses of variance followed by Tukey’s HSD tests (α=.05). With the 3H-thymidine incorporation assay, no significant differences (P >.05) were found between the materials. All acrylic resins were graded by the 3H-thymidine incorporation assay as moderate cytotoxic. Cytotoxicity as assessed by the MTT showed no significant differences among materials (P > .05). When the cytotoxicity was compared with control group, all resins assay were in the borderline between non cytotoxic and slight cytotoxic by the MTT. The incorporation of cross-linking agents, EGDMA and BDMA had no significant influence on the cytotoxicity observed
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