Isolation and Purification of Fibrino(geno)lytic Enzymes from Agkistrodon Blomhoffi Usuriensis Venom


(*) Corresponding author


Authors' affiliations


DOI's assignment:
the author of the article can submit here a request for assignment of a DOI number to this resource!
Cost of the service: euros 10,00 (for a DOI)

Abstract


This paper describes the purification of fibrino(geno)lytic enzymes from Agkistrodon blomhoffii ussuriensis venom in three chromatographic stages. Two single-stranded enzymes with molecular weight of 30 and 37 kDa were separated. Both toxins were purified using affinity, hydrophobic interaction and ion-exchange chromatography and were identified as metaloproteinase and serine protease respectively
Copyright © 2012 Praise Worthy Prize - All rights reserved.

Keywords


Snake Wenom; Fibrino(Geno)Lytic Enzyme; Chromatography

Full Text:

PDF


References


F. Swenson S, Jr. Markland, Snake venom fibrin(ogen)olytic enzymes, Toxicon 45 (2005) 1021 – 1039.

A. Leonardi, J. Fox, A. Trampus-Bakija et al., Ammodytase, a metalloprotease from Vipera ammodytes ammodytes venom, possesses strong fibrinolytic activity, Toxicon 49 (2007) 833 – 842.

M. Samel, J. Subbi, J. Siigur et al., Biochemical characterization of fibrinogenolytic serine proteinases from Vipera lebetina snake venom, Toxicon 40 (2002) 51 – 54.

C. Bernardes, N. Santos-Filho, T. Costa et al., Isolation and structural characterization of a new fibrin(ogen)olytic metalloproteinase from Bothrops moojeni snake venom, Toxicon 51 (2008) 574 – 584.

F. Markland, Snake venoms and the hemostatic system, Toxicon 36 (1998) 1749 – 1800.

S. Swenson, C. Toombs, L. Pena et al. -Fibrinogenases, Current Drug Targets Cardiovasc Haematol Disord. 4 (2004) 417 – 435.

S. Deitcher, C. Toombs, Non-clinical and clinical characterization of a novel acting thrombolytic: alfimeprase, Pathophysiol Haemost Thromb 34 (2005) 215 – 220.

M. Bolger, S. Swenson, F. Markland, Three-dimensional structure of fibrolase, the fibrinolytic enzyme from southern copperhead venom, modeled from the X-ray structure of adamalysin II and atrolysin C, AAPS Pharm Sci. 3 (2001) E16.

S. Swenson, L. Bush, F. Markland, Chimeric derivative of fibrolase, a fibrinolytic enzyme from southern copperhead venom, possesses inhibitory activity on platelet aggregation, Arch Biochem Biophys. 384 (2000) 227 – 37.

E. Sanchez, L. Bush, S. Swenson, F. Markland, Chimeric fibrolase: covalent attachment of an RGD-like peptide to create a potentially more effective thrombolytic agent, Thromb Res. 87 (1997) 289 – 302.

V.L. Karbovskiy, T.N. Platonova, O. V. Gornitskaya et al., Fibrino(geno)lytic enzyme from Agkistrodon blomhoffi usuriensis snake venom: isolation, partial characterization and influence on platelets aggregation, Biotechnology 3 (2010) 35 – 40.

L. Ostapchenko, O. Savchuk, N. Burlova-Vasilieva, Enzyme electrophoresis method in analysis of active components of haemostasis system, Advances in Bioscience and Biotechnology 2 (2011) 20-26.


Refbacks

  • There are currently no refbacks.


Please send any question about this web site to info@praiseworthyprize.com
Copyright © 2005-2024 Praise Worthy Prize